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The column was then washed with 20 CV of DPBS/0.05% (v/v) sodium azide, followed by elution with 9 CV of 2 M MgCl 2 (Sigma-Aldrich)/20 mM Tris-HCl (Sigma-Aldrich) buffer (pH 7.4) at a flow rate of 2 mL/min

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Hepatocyte Release of HMGB1 During Acetaminophen-Induced Liver Injury and Deleterious Feed-Forward Process: Implication of Necroptosis Through TRIF/RIPK3 Axis

doi:10.1002/mnfr.201200646

"Decline in transcriptional activity of Nrf2 causes age-related loss of glutathione synthesis." Proc Natl Acad Sci